Our Services

Our Record of Success

In the Past 18 months:
  • Transgenic: average of 4 founders per project from over 20 projects
  • Gene targeting: Out of 14 projects (some still on going), 6 have gone germline and 4 more have positive clones confirmed and ready to inject.
  • Embryo and Sperm Cryopreservation: 116 lines frozen down: 100% QC success (live pups of proper genotype recovered from freeze) for frozen embryos and 85% for frozen sperm.
  • Rederivation: delivered an average of 12 pups for 33 lines.

Does My Mouse Already Exist?

Mouse Production

Gene Targeting through Homologous Recombination in ES cells
  • Electroporation of targeting construct
  • Cassette Deletion – In vitro deletion of drug selection cassettes (or other target sequences) using either Cre or Frt recombinase
  • Injection of ES cells into blastocyst stage embryos for production of chimeras
Gene Editing through CRISPR
  • Injection of Crisper/CAS RNA (and donor DNA if applicable) for gene editing in the 1-cell embryo for production of mutant mouse lines.
Transgenics
  • Injection of plasmid or BAC DNA construct into 1-cell embryo to produce novel Transgenic mouse lines

Cryopreservation

Sperm Cryopreservation
Embryo Cryopreservation
  • Via IVF using 1-2 males containing desired allele(s) and wildtype females
  • Via natural matings using PI’s males and females containing desired allele(s)

Rederivation

Importing frozen sperm or embryos to rederive a mouse line on campus
Importing live mice for rederivation via IVF
Rederiving to “clean” mouse line of pathogens (for example, to bring a line into the HSRB facility)

Exporting Mice

Sperm Cryopreservation
Embryo Cryopreservation
  • Via IVF using 1-2 males containing desired allele(s) and wildtype females
  • Via natural matings using PI’s males and females containing desired allele(s)
Rederive to “clean” mouse line of pathogens to send live mice to collaborator

Other Services

Teratoma Formation
Timed Mating and Embryo Collection
Contract Service
Frozen embryo/sperm storage
Tail Biopsy DNA Purification