Microvolution
Microvolution is deconvolution software capable of processing images at orders of magnitudes faster than CPU-based software. The blind deconvolution option can give more accurate results in scenarios where the theoretical PSF is inaccurate. Batch processing is available, and output options now include OME-TIFF and the pyrmidal Imaris .ims file types.
How to access?
Threee different analysis workstations with CUDA-capable GPUs have Microvolution.
Book Microvolution with the desired workstation (e.g. "Workstation 0 + Microvolution") to avoid scheduling conflicts as only one person can use Microvolution at a time.
Get started with the official Microvolution manual or submit a training request for targeted training.
Pinhole calculations for confocal microscopes
Why is this necessary? Find out more at General Backprojected Pinhole Calculator
| Point Scanning Confocal Microscope | Pinhole shape | Immersion oil RI (at 23°C) |
|---|---|---|
| Nikon A1R HD25 | hexagonal | 1.515 |
| Olympus FV1000 | square | 1.518 |
| Leica SP8 | square | 1.518 |
| Spinning Disk Unit | Physical diameter [μm] | Pinhole spacing [μm] | RI of immersion oil (at 23°C) |
|---|---|---|---|
| CrestOptics X-Light V2 LFOV | 50 | 250 | 1.515 |
If using Microvolution with images acquired from another spinning disk, you may find SVI's YokogawaDisk page to be helpful.