The Facility Line STED microscope from Abberior Instruments has four lasers for excitation (405, 485, 561, 640 nm) as well as two pulsed STED lasers (595 and 775 nm). Patented adaptive illumination methods enable live-cell imaging with drastically reduced photobleaching and phototoxicity. FLIM capabilities available through two channel TCSPC.
- STED super-resolution microscope on an inverted Olympus IX83 body
- Two pulsed STED lasers (595 and 775 nm) complement four excitation lasers (405, 485, 561, 640 nm)
- Okolab stage top incubator for CO2 and temperature control (Uno-T line)
- Multi-channel, z-stacks, time-lapse imaging, multi-point capture, tiling
- Z-drift compensation unit
- FLIM, FRAP, FLIP, FRET, photoactivation, colocalization
- Software options include: SymPhoTime 64--Picoquant data acquisition and analysis software
Objectives
Excitation laser lines (nm)
405 - CW, 20 mW
485 - ~1mW pulsed
561 - ~200 µW pulsed
640 - ~1mW pulsed
Pulsed STED lasers (nm)
595 - up to 400 mW
775 - >2750 mW @ 40 MHz, repetition rate 25-40 MHz
Detectors
Four ultra-sensitive single-photon-counting APDs with tunable detection bands between 400 and 800nm
Stage
IX3-SSU ultrasonic xy-scanning stage for IX83
Autofocus
Custom design based on Olympus IX3-ZDC-12 z-drift compensation unit for steady confocal/STED imaging
Stage top incubator
Okolab, Uno-T line with temperature control up to 40°C
- Chat with us regarding labelling--especially if trying to push resolution limits
- Adaptive illumination via RESCue, DyMIN, and MINFIELD
Stoyan Ivanov stoyan.ivanov@emory.edu