The Emory HPLC Bioanalytical Core (EHBC), one of the Emory Integrated Core Facilities (EICF), is supported by the Department of Pharmacology and Chemical Biology, the Georgia Clinical & Translational Science Alliance, and the Emory University School of Medicine. The HPLC Bioanalytical Core measures monoamines, purines, and amino acids using HPLC methods in biological matrices including tissue extract, cell lysate, plasma, serum, CSF, urine, microdialysate. In addition, we provide expertise to develop and validate HPLC method to measure some drugs, endogenous compounds, peptides, and proteins. Please contact us to discuss your experimental needs.
The HPLC bioanalytical core offers the ability to identify and quantify monoamines, amino acids, purine bases, and nucleotides from a variety of biological samples. We also provide expertise to develop and validate HPLC methods. Services provided:
- Monoamine analyses using HPLC coupled to electrochemical detector. Analytes include dopamine, DOPAC, norepinephrine, HVA, 3-MT, 5-HIAA, MHPG, serotonin
- Amino acid analyses using pre-column OPA derivatization HPLC coupled to electrochemical detector. Analytes include glutamate, glutamine, alanine, glycine, arginine, taurine, GABA, and tyrosine
- Purine analyses using HPLC coupled to photodiode array detector. Analytes include ATP, ADP, AMP, adenosine, adenine, GTP, GDP, GMP, guanosine, hypoxanthine, inosine, xanthine and uric acid
- We also offer custom analyses to develop and validate HPLC methods. Some examples such as small molecule drugs, purines, pyrimidines and related molecules, electrochemically active compounds, neuromodulators, their precursors and metabolites
Submit a Project
The High Performance Liquid Chromatography Bioanalytical Core (HPLC) is one of the Emory Integrated Core Facilities (EICF). We use PPMS to provide access to our services and track your projects. Please click here if you need an account established for your use.
PPMS - Access Services
Please email us at HPLC@Emory.edu