Grant Materials
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The Emory HPLC Bioanalytical Core (EHBC), one of the Emory Integrated Core Facilities (EICF), is supported the Georgia Clinical & Translational Science Alliance, and the Emory University School of Medicine. The HPLC Bioanalytical Core measures monoamines, purines, and amino acids using HPLC methods in biological matrices including tissue extract, cell lysate, plasma, serum, CSF, urine, microdialysate. In addition, we provide expertise to develop and validate HPLC method to measure some drugs, endogenous compounds, peptides, and proteins.
Emory HPLC Bioanalytical Core (EHBC) is located at the Woodruff Memorial Building Room 6306 and occupies 400 sq.ft of space. The core is equipped with five UPLC/UHPLC/HPLC systems, which includes one ACQUITY UPLC H-Class PLUS System, one ACQUITY UHPLC Arc System, two ESA CoulArray detection systems and one Waters HPLC system. The Core also has access to additional equipment for sample preparation including a SpectraMax M5e spectrophotometer (Molecular Devices, Sunnyvale CA) which is a UV/Vis variable wavelength microtiter plate reader with Softmax Pro software used for performing colorimetric spectrophotometrical protein assays, and Branson sonifier 450 which is used for tissue homogenization. Also available are a Millipore Water system, centrifuges, sonicator, pH meters, balances, oven, refrigerators, hot plates, stirrers, -70˚C freezers, and other standard equipment.
Emory HPLC Bioanalytical Core (EHBC) is located at the Woodruff Memorial Building Room 6306 and occupies 400 sq.ft of space. The core is equipped with one ACQUITY UPLC H-Class PLUS System, one ACQUITY UHPLC Arc System, two ESA CoulArray systems and one Waters HPLC system.
ACQUITY UPLC H-Class PLUS System. The ACQUITY UPLC H-Class PLUS Core System includes integrated solvent, sample and single column heater (CH-A) configured for quaternary solvent delivery (QSM) and sample introduction via a direct inject sample manager (SM-FTN-H). The system was equipped with both ACQUITY UPLC PDA eLambda Detector, ACQUITY UPLC Fluorescence Detector. The system is controlled by Waters Empower 3.0 software.
ACQUITY UHPLC Arc System. The ACQUITY Arc System with Temperature Control Configuration includes quaternary solvent delivery (QSM-R) and sample introduction via a direct inject sample manager (SM FTN-R) with temperature control. The system was equipped with 2998 PDA w/ Low Dispersion Flowcell and electrochemical detector. The system is controlled by Waters Empower 3.0 software.
Two ESA CoulArray Systems. Each of the ESA 5600A CoulArray systems is equipped with ESA model 584 pump, PEEK Pulse damper, ESA 542 refrigerated autosampler injector, temperature controller, model 5020 guard cell, and model 6210 coulometric array cell. The system is controlled by ESA CoulArray V3.10 software.
Waters HPLC System. Waters HPLC system consists of model 717-plus autosampler, model 1525 binary pump, model 2996 photodiode array detector, fraction collector and 2465 electrochemical detector. The system is controlled by Empower 2.0 software.
Additional Equipment Access to:
SpectraMax M5e spectrophotometer. A UV/Vis variable wavelength microtiter plate reader with Softmax Pro software used for performing colorimetric spectrophotometrical protein assays.
Branson sonifier 450. A sonicator used for tissue homogenization.