Analysis
Use our powerful workstations to access proprietary and open-source image analysis/processing software.
Licensed Software
Imaris enables visualization of 3D, 4D and 5D data sets via multiple volume rendering algorithms and quantitative analysis.
Licensed packages include
- Tracking (motion analysis, cell division and lineage)
- FilamentTracer (Neuron tracing, spine detection, and measurements)
- ImarisCell which allows for analysis of cells and their components
- Colocalization (Pearson's correlation coefficient, Mander's coefficient)
FilamentTracer is available at workstation 4 in Whitehead and workstation 5 in HSRB II.
ImarisCell is available at workstation 0 in Winship and workstation 3 in HSRB.
For more information please reach out to Stoyan (Stoyan.ivanov@emory.edu)
Deconvolution software which is capable of processing images at orders of magnitudes faster than CPU-based software through the use of NVIDIA GPUs. Its "blind" deconvolution feature allows it to capture unmodeled aberrations such as coma without an experimentally measured PSF.
What does deconvolution do?
It increases your image's contrast / signal-to-noise ratio which will be helpful for quantification (segmentation, colocalization, intensity measurements, etc.). When widefield datasets are deconvolved, the blur from out-of-focus fluorescence can be suppressed.
Gen5 is available on select workstations and is intended to be used in conjunction with our BioTek Lionheart FX.
Its analysis options focus on 2D datasets, so z-stacks will need to be projected into one plane.
Workstations
Remote access is available for our workstations. Contact us if you're unsure which is best suited for you!
Workstation 0
License(s): Imaris for Cell Biologists, Microvolution
- Location: WCI 4th floor labs, bench 19
- RAM: 512 GB
- GPU: NVIDIA Quadro M6000 24GB
- CPU: 2x Intel Xeon E5-2667 v4 @ 3.2 GHz
Workstation 2
License(s): Volocity
- Location: WCI 4th floor labs, bench 19
- RAM: 64 GB
- GPU: AMD Radeon HD 6950
- CPU: Intel Xeon E5-2650 v3 @ 2.30 GHz
Workstation 3
License(s): Imaris for Cell Biologists, Microvolution, Gen5
- Location: HSRB EG21
- RAM: 64 GB
- GPU: NVIDIA Quadro M5000
- CPU: Intel i7-6700K @ 4.00 GHz
Workstation 4
License(s): Imaris for Neuroscientists, Microvolution, Gen5
- Location: WBRB 421
- RAM: 64 GB
- GPU: NVIDIA 1070 Ti
- CPU: AMD Ryzen 7 2700X @ 3.7 GHz (Boost to 4.3 GHz)
Workstation 5
License(s): Imaris for Neuroscientists
- Location: HSRB II, S-305
- RAM: 512 GB
- 2.9 GHz, 64 core processors
- 20 TB hard drive for data and analysis