Miltenyi UltraMicroscope Blaze Lightsheet
A fully automated light sheet microscope for imaging large or multiple cleared samples at subcellular resolution.
- Fully automated
- large sample chamber, holds 500mls of imaging solution
- Uni- and bi-directional illumination with 1-6 sheets
Laser Lines:
Excitation Emmision
405 460/40
488 525/50
561 620/60
640 680/30
785 850lp
| Objective lenses | 1.1× | 4× | 12× | |
| Total magnification | 0.66–2.75× | 2.4–10× | 7.2–30× | |
| Numerical aperture | 0.1 | 0.35 | 0.53 | |
| Max. theoretical resolution at detector | 4.8 µm | 1.3 µm | 0.5 µm | |
| Working distance | ≤17 mm | ≤16 mm | ≤10.9 mm | |
| Detector | 4.2 Megapixel sCMOS camera | |
| Active pixels (w × h) | 2048 × 2048 | |
| Pixel size | 6.5 µm × 6.5 µm | |
| Sensor size | 13.3 mm × 13.3 mm; 18.8 mm diagonal | |
| Readout noise | 0.8 med e- | |
| Maximal frame rate | 100 fps | |
| Maximum quantum efficiency | 82% |
- We prefer the use of the iDISCO clearing method as we are set up with the ECI for correct RI matching however, other methods such as Clarity can be used.
- Please reach out to April Reedy for a consultation first to plan your experiment.
Primary contact
April Reedy, PhD april.reedy@emory.edu