Nikon N-SIM super-resolution

The Nikon Structured Illumination super-resolution Microscope has two laser lines (488nm and 560nm) and is capable of 2D-SIM, 3D-SIM, and TIRF-SIM.

Book me about Nikon N-SIM super-resolution

The Nikon Structured Illumination (super-resolution) Microscope can beat the resolution of conventional microscopes by a factor of two in all directions and is capable of TIRF-SIM acquisitions.

  • Linear 3D-SIM ideally leads to resolutions of ~120nm in xy, ~300nm in z
  • Microscope body: Inverted Nikon Eclipse Ti
  • Modalities: widefield, 2D-SIM, 3D-SIM, TIRF-SIM
  • piezo z-stacks, time-lapse imaging, tiling
  • Perfect Focus System
  • Environmental chamber for live cell: Tokai Hit INUG2AH-TIZSH 

Objectives

Laser lines (nm)

Camera

2D-SIM up to 3um deep

3D-SIM up to 20um deep

Acquisition software: NIS-Elements v5.0.2

Sample prep and image reconstruction guide

Primary contact
Mark (Shaojin) You     syou2@emory.edu