The "Crest" has seven laser lines from blue to NIR for fast, large field of view confocal imaging in many channels. Two water immersion objectives facilitate high-resolution imaging in gels (Matrigel, hydrogel, low concentration agarose) and aqueous media.
- Modalities: spinning disk confocal, brightfield, DIC, widefield
- piezo z-stacks, time-lapse imaging, tiling
- Perfect Focus System
- Seven laser lines for fluorophores like: DAPI, CFP, GFP, YFP, RFP, Cy5, Cy7
- Air, water immersion, and oil immersion objectives
- Environmental chamber for live cell: Tokai Hit STXF-TIZWX-SET
- Universal sample holders
- Microscope body: motorized inverted Nikon Eclipse Ti2-E
- hardware triggering for multicolor imaging without switching emission filters
- Software: Nikon JOBS, General Analysis 3
Objectives
Laser lines (nm) from Lumencor CELESTA
Camera
Hamamatsu Flash 4.0 sCMOS C11440-22C
Filters
Spinning Disk Unit
Crest X-Light V2 L-FOV (Large-Field of View)
- 50um pinhole size with 250um pinhole spacing
- Do not use ibidi polymer slides with the Nikon immersion oil.
- Additional objectives: 20x ELWD, 100x/1.49 Apo TIRF