Nikon CrestOptics Spinning Disk


The Crest X-Light V2 L-FOV spinning disk unit paired with a Nikon Ti2-E inverted microscope enables fast and gentle confocal imaging with a large field of view.

Book me about Nikon CrestOptics Spinning Disk

The "Crest" has seven laser lines from blue to NIR for fast, large field of view confocal imaging in many channels. Two water immersion objectives facilitate high-resolution imaging in gels (Matrigel, hydrogel, low concentration agarose) and aqueous media.

  • Modalities: spinning disk confocal, brightfield, DIC, widefield
  • Piezo z-stacks, time-lapse imaging, tiling
  • Perfect Focus System
  • Seven laser lines: 365 nm, 440 nm, 488 nm, 514 nm, 561 nm, 640 nm
  • Air, water immersion, and oil immersion objectives
  • Environmental chamber for live cell: Tokai Hit STXF-TIZWX-SET
  • Universal sample holders
  • Microscope body: motorized inverted Nikon Eclipse Ti2-E
  • Hardware triggering for multicolor imaging without switching emission filters
  • Software: Nikon JOBS, General Analysis 3

Objectives

Laser lines (nm) from Lumencor CELESTA

Camera

Hamamatsu Flash 4.0 sCMOS C11440-22C

Filters

FPbase

Spinning Disk Unit

Crest X-Light V2 L-FOV (Large-Field of View)

- 50um pinhole size with 250um pinhole spacing

  • Do not use ibidi polymer slides with the Nikon immersion oil.
  • Correction collars on 20x/0.45 ELWD, 20x/0.95 Water, 40x/1.15 Water, 60x/1.49 Oil, and 100x/1.49 Oil objectives
Additional Objectives

Primary Contact

Harold (Hai) Vo, PhD     hai.vo@emory.edu