Nikon CSU-W1 SoRa Spinning Disk
The CSU-W1 SoRa is a confocal field scanning spinning disk system and a confocal super resolution system in a single package. We offer dry as well as water immersion lenses for high resolution imaging with fixed and live cell.
- Modalities: spinning disk confocal, brightfield, widefield
- piezo z-stacks, time-lapse imaging, tiling
- Perfect Focus System
- Four laser lines for fluorophores like: DAPI, CFP, GFP, YFP, RFP, Cy5,
- Air, water immersion, and oil immersion objectives
- Environmental chamber for live cell: Tokai Hit STXF
- Universal sample holders
- Microscope body: motorized inverted Nikon Eclipse Ti2-E
- hardware triggering for multicolor imaging without switching emission filters
- Software: Nikon JOBS, General Analysis 3
Laser Lines
385, 475, 594, and 621
OBJECTIVES
| CFI60 PLAN APOCHROMAT LAMBDA D 4X |
| CFI60 PLAN APOCHROMAT LAMBDA D 20X |
| CFI APO LWD 40X WI 1.15 NA LAMBDA S |
| CFI60 PLAN APOCHROMAT LAMBDA D 60X Oil |
| CFI60 PLAN APOCHROMAT LAMBDA D 100X Oil |
Camera
Hamamatsu ORCA-Fusion Gen III sCMOS
Filters
| ET455/50M, DAPI-ET EMISSION FILTER 25MM |
| ET525/36M EMISSION FILTER, 25MM |
| ET605/52M EMISSION FLTER 25MM |
| 89101M EM FLTR DAPI/FITC/TRITC/CY5, 25MM |
Spinning Disk Unit
CSU-W1 DL DSK SNGL CAM 50/50UMSR LSR SYS
Microlensing the emission pinhole at the correct magnification counteracts the mismatch between an on-axis excitation PSF and the effective confocal PSF (product of excitation and emission PSFs) through an emission pinhole that is not infinitely small.
By microlensing, individual points’ divergence angle onto the pinhole is reduced 2x, mimicking the effect of an infinitely small ideal pinhole, but not compromising signal brightness.
- Do not use ibidi polymer slides with the Nikon immersion oil.
Hunter Hakimian hunter.hakimian@emory.edu