Facilities, other resources and equipment

To request a letter of support, please review the EICF Core Letter of Support Request process and complete the Request Form at least 2 weeks before the grant proposal due date.

Facilities & Other Resources

Updated: 11 February 2025

Specific Fields Relevant for Mouse Transgenic and Gene Targeting Core (TMF) Users

Mouse Transgenic and Gene Targeting Core (TMF)

The Mouse Transgenic and Gene Targeting Core (TMF), one of the Emory Integrated Core Facilities (EICF), provides state-of-the-art equipment and expertise for making genetically engineered mouse models, moving alleles on or off-campus, and cryopreserving existing strains of mice. The TMF is located on the ground floor of the Emory Health Sciences Research Building-1 (HSRB1) and occupies laboratories V202, V206, V208, and V209. The laboratory space includes a fully equipped tissue culture suite dedicated to embryonic stem (ES) cell culture, an embryology laboratory, a microinjection/embryo manipulation space, a surgical suite, a molecular biology space, and a cryopreserved embryo/sperm archival space. The TMF has 100 sq ft of dedicated office space on the 1st floor of HSRB1, which provides space for meeting customers and TMF team meetings. A dedicated workspace for TMF staff is also located in the HSRB animal facility. The TMF lab is supported by three staff members. The Core Director (Dr. Karolina Nitsche) and Scientific Director (Dr. Tamara Caspary) provide grant application support and project consultation.

The animal holding/breeding room located in V210 is part of the animal facility and is operated by the Emory Division of Animal Resources (DAR) as detailed below. All of the mice used by the core are housed in this facility. It is managed by DAR in strict compliance with the standards and policies of the Public Health Service. Mice are housed in a ventilated auto-water caging system and are changed under laminar flow hoods. The DAR staff are responsible for daily care of the animals as well as health monitoring and surveillance. The DAR also maintains vigorous quarantine and sentinel programs.

The core facility is fully equipped to perform all experimental procedures associated with the production of genetically modified mice. In addition, it has research space and equipment to assist laboratories with auxiliary procedures for the production and analysis of genetically modified mice. These include rederivation and cryopreservation services, as well as custom mouse needs. The TMF now has several services available in rats, including sperm and embryo cryopreservation and semen evaluation.

Major Equipment

Updated: 11 February 2025

Major Equipment relevant for Mouse Transgenic and Gene Targeting Core (TMF) Users

Mouse Transgenic and Gene Targeting Core (TMF)

The Emory Mouse Transgenic and Gene Targeting Core (TMF), one of the Emory Integrated Core Facilities (EICF), provides state of the art equipment and expertise for making genetically engineered mouse models, moving alleles on or off campus, and cryopreserving existing strains of mice. With laboratories and animal facilities located on the ground floor of the Health Sciences Research Building-1 (HSRB-1), the TMF provides rodent-related services that are supported by the following infrastructure:

Microinjection/Gene Editing (V206)
Microinjection Station #1:
  • Leica DMi8 Inverted microscope with Phase contrast and DIC optics
  • Narishige manual micromanipulators
  • Eppendorf microinjectors
  • Eppendorf FemtoJet 4i transjector
  • Prime Tech PMM6G Piezo Impact Drive
  • Brook Industries heating/cooling Thermal Stage System
Microinjection Station #2:
  • Leica DMi8 Inverted microscope with Phase contrast and DIC optics
  • Narishige manual micromanipulators
  • Eppendorf microinjectors
  • Eppendorf FemtoJet 4i transjector
  • Brook Industries heating/cooling Thermal Stage System
Embryo collection, manipulation, and rederivation (V206)
  • Leica M80 Binocular Stereo Microscope with heating stage (x2)
  • Leica M60 Binocular Stereo Microscope
  • Panasonic CO2 Incubator (x2)
  • Sutter P-1000 Micropipette puller
  • BTX Electroporation system
ES Cell Culture (V208)
  • Nuaire Class II Biological Safety Cabinet
  • ThermoScientific centrifuge
  • Eppendorf 5430R Refrigerated Centrifuge
  • Panasonic CO2 Incubator
  • Leica DMi1 Inverted Microscope
  • BioRad Electroporation Unit
Sperm/Embryo Cryopreservation (V202, V206, V208)
  • FTS Bio-Cool Rate Freezer BC-V-40A
  • Locator 4 Liquid Nitrogen Dewar
  • MVE Liquid Nitrogen Dewars (x11)
  • CEROS-2 Animal Sperm Analyzer
Molecular Biology (V209)
  • Ohaus Explorer Analytical Balance
  • Eppendorf Centrifuge 5415 C
  • Thermo Scientific Proflex 3X32-WELL PCR System
  • ABI 9700 thermal cycler (x2)