Facilities, other resources and equipment
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Facilities & Other Resources
Updated: 1 September 2020
Specific Fields Relevant for Mouse Transgenic and Gene Targeting Core (TMF) Users
Other:
Mouse Transgenic and Gene Targeting Core (TMF)
The Mouse Transgenic and Gene Targeting Core (TMF), one of the Emory Integrated Core Facilities (EICF), provides state of the art equipment and expertise for making genetically engineered mouse models, moving alleles on or off campus, or cryopreserving existing strains of mice. The TMF is located on the ground floor of Emory Health Sciences Research Building and occupies laboratories EG41 and EG42. The EG42 space includes a fully equipped tissue culture suite dedicated to embryonic stem (ES) cell culture, and microinjection/embryo manipulation space, and surgical suite. The EG41 space includes molecular biology space, and cryopreserved embryo/sperm archival space. The TMF has 100 sq ft of dedicated office on the 1st floor of the Health Sciences Research Building, which provides for meeting customers, and TMF team meetings. Dedicated workspace for TMF staff is also located in HSRB animal facility.
The animal holding/breeding room is located in EG57 is part of the animal facility and is operated by the Division of Animal Resources (DAR) as detailed below. All the mice used by the core for the production of gene edited mice are housed in this facility. It is managed by the Emory University Division of Animal Resources (DAR) in strict compliance with the standards and policies of the Public Health Service. Mice are housed in ventilated auto-water caging system and are changed under laminar flow hoods. The DAR staff is responsible for daily care of the animals as well as health monitoring and surveillance. The DAR also maintains vigorous quarantine and sentinel programs.
The core facility is fully equipped to perform all experimental procedures associated with the production of gene edited mice. In addition, the core facility has research space and equipment to assist laboratories with auxiliary procedures for the production and analysis of transgenic and gene targeted mice. These include rederivation and cryopreservation services, and custom mouse needs.
Major Equipment
Updated: September 1, 2020
Major Equipment relevant for Mouse Transgenic and Gene Targeting Core (TMF) Users
Mouse Transgenic and Gene Targeting Core (TMF)
The Mouse Transgenic and Gene Targeting Core (TMF), one of the Emory Integrated Core Facilities (EICF), provides state of the art equipment and expertise for making genetically engineered mouse models, moving alleles on or off campus, or cryopreserving existing strains of mice. With laboratories and animal facilities located on the ground floor of the Health Sciences Research Building (HSRB), the TMF provides mouse-related services that are supported by the following infrastructure:
Microinjection/Gene Editing (EG42):
Microinjection Station #1:
- Leica DMi8 Inverted microscope with Phase contrast and DIC optics
- Narishige manual micromanipulators
- Eppendorf microinjectors
- Eppendorf FemtoJet 4i transjector
- Prime Tech PMM4G Piezo Impact Drive
- Brook Industries heating/cooling Thermal Stage System
Microinjection Station #2:
- Leica DMi8 Inverted microscope with Phase contrast and DIC optics
- Narishige manual micromanipulators
- Eppendorf microinjectors
- Eppendorf FemtoJet 4i transjector
- Brook Industries heating/cooling Thermal Stage System