Services

The EIMLC offers the ability to identify and quantify lipid species (e.g. fatty acids, phospholipids, ceramides, sphingosines, cholesterols, bile acids) and metabolites (e.g. amino acids, TCA intermediates, organic acids) from a variety of tissue types. We also offer custom lipidomic and metabolomic analyses to fit your research needs.

Services include:

• Sample preparation via automated and robust sample handling instrument manifold (Biotage Extrahera, Uppsala Sweden). Traditional lipid extraction protocols using organic solvents, i.e. Bligh and Dyer, MtBe Method, and others performed upon request.

• High resolution, untargeted lipidomics analyses combining liquid chromatography with ion mobility high-sensitivity, high-resolution mass spectrometer. This LC-MS approach surveys lipids in the submitted sample by exact mass, collision cross section (ccs) values, and product ion spectra to produce high confidence identifications of species.

• Targeted lipidomics analyses via tandem mass spectrometry to specifically target phospholipids classes, low abundance lipid classes, and/or signaling lipids by precursor ion scanning or multiple reaction monitoring (MRM) based assays. Identified species are quantified through the use of external calibration curves or labeled internal standards. Targeted assays include:
  • CoAs
  • Carnitines
  • Oxylipins/Endocannabinoids
  • Bile Acids
  • Nonesterified Fatty Acids (Short and long chain fatty acids)
  • TCA metabolites
  • Sterols

• Semi-quantitative untargeted and targeted metabolomics (quantitative) analyses using high resolution LC/MS to characterize lipid and soluble metabolites in submitted samples.

• Customized analysis to quantify drug/drug metabolites provided upon request.

• Consultation for experimental design for ‘omics projects, proper handling of lipid samples, and/or data interpretation.

• Data integration services are offered to produce publication quality figures of ‘omics data. This includes heat maps, volcano plots, WGCNA, etc. Integration of lipid/metabolomics datasets with clinical data.

EIMLC Rate Table

Questions?

Contact us at 404.727.3193 or kmaners@emory.edu