Resources

General

How to use FlowJo off-campus using an Emory VPN connection

Follow these instructions to create and open a VPN (Virtual Private Network) connection to the Emory system.

Then fire up FlowJo (make sure you've registered FlowJo!). Let us know if you have any questions.

Protocols and Procedures

• Antibody Titering ( Carleton Stewart, et al)
• Immunophenotyping (Carleton Stewart, et al)
• Cell Cycle Staining Protocol  (special Thanks to Dr. Ritu Aneja and the Harish Joshi Lab)
• A WinMDI Tutorial
• A Setup System for Compensation: BD CompBeads plus BD FACSDiva Software (pdf)
• Determining Antibody Titers (special Thanks to Dr. Carleton Stewart and RPCI)
• Titering Directly Conjugated Antibodies to Extracellular Antigens (special Thanks to Dr. Carleton Stewart and RPCI)
• Apoptosis Prep for Training
• Extracellular Immunophenotyping prep (special Thanks to Hilary Rosenthal, MT (ASCP) and the Waller Lab)
• Intracellular Staining prep (special Thanks to Hilary Rosenthal, MT (ASCP) and the Waller Lab)
• Cell-cycle protocol for yeast (special Thanks to Missy Brykailo and the Fridovich-Keil Lab)

Training Materials

• BD FACSymphony™ A3 Flow Cytometer User’s Guide
• BD FACSymphony™ A5 SE Flow Cytometer User's Guide
• Cytek Aurora User’s Guide

Sorting

In order to avoid the common pitfalls inevitable in preparing cells for sorting, we recommend reading the following links:

• Guide to Getting a Great Sort
• Sort Buffer Selection
• BD FACService Technotes, Vol 9 No. 4, Oct 2004

Sorting Presentations

• How a sorter works:

References

1. Flow Cytometry: A Practical Approach. Edited by MG Ormerod. (IRL Press, Oxford. 1994. ISBN 0-19 963461-0)
2. Practical Flow Cytometry. 3rd Edition. Howard M Shapiro. (Alan R Liss, Inc. ISBN 0-471-30376-3).
3. Flow Cytometry. First Principles. Alice Longobardi Givan. (Wiley-Liss, New York, 1992. ISBN 0-471-56095-2.)
4. Handbook of Flow Cytometry Methods. Edited by J Paul Robinson. (Wiley-Liss, New York, 1993. ISBN 0-471-59634-5.)

The Emory School of Medicine Core Facility for Flow Cytometry (EFCC) provides analysis and sorting services and experimental/panel design to the research community in & around Emory University Campus (Emory, Georgia Tech, & UGA). Established in 2001, the SOM Flow Core offers a wealth of experience & knowledge relative to Flow Cytometry.

Centrally Located within the heart of Emory’s Health Science & Research Quad (O. Wayne Rollins Research Center, Whitehead Biomedical Research, Emory Vaccine Center, Rollins School of Public Health & HSRBII), we offer a convenient location staffed with dedicated professionals with decades of experience.

Instrumentation

Analysis:

• FACSymphony S6 SE*, FACSDiscover S8* (Becton Dickinson)
• Aurora CS* (Cytek)
• 24+ Fluorescence Detectors
•  Lasers
  • UV (355 nm)
  • Violet (405 nm)
  • Blue (488 nm)
  • Green (561 nm)
  • Red (640 nm)