Microarray Analysis of Glycan Microarrays

Microarray Analysis of Glycan Microarray

We have many years of experience analyzing defined, as well as, shotgun glycan microarrays. Glycans microarrays are produced either by outside sources or in our core EGMIC. EGMIC staffs can carry out assays using your glycan binding proteins (GBP) or organism(s) and assist in interpretation of data using bioinformatic software developed in house.

EGMIC will assist Emory Investigators with making requests for the glycan microarray available in National Center for Functional Glycomics at Harvard Medical School in Boston.
  1. NCFG slides from the Consortium for Functional Glycomics contains > 500 defined glycans.  These glycan structures are mostly synthetic glycans, many of which occur in mammalian animals.   
  2. Microbial glycan microarray (MGM) slides from the Consortium for Functional Glycomics contains a diverse set of microbial glycans isolated from a broad range of microbial genera. 
  3. Human milk glycans array.  This glycan microarray include many human milk glycan structures (~200 fractions).
The following glycan arrays are developed and produced by our core (EGMIC) in collaboration with the National Center for Functional Glycomics at Harvard Medical School in Boston.   
  1. Sialylated oligosaccharides array containing the major ganglioside glycans and a variety of other sialic acid-containing glycans from natural sources.  This glycan microarray is comprised of approximately 50 sialylated oligosaccharides.   
  2. Hyaluronic acid oligomer array. Hyaluronic acid was digested with bovine testicular hyaluronidase to small oligomers (total 20 unique glycans).   
  3. Glycosaminoglycan array (GAG).  This glycosaminoglycan microarray is comprised of oligosaccharide fragments (~200 fractions), digested and purified from hyaluronic acid, chondroitin sulfate and heparin.  The detailed structures of each glycan are yet to be defined as the array is still in development.
In addition, we have following array from another source.
  1. ABH blood type I-VI antigens array.  This glycan microarray array presents the glycan determinants representing the human blood group structures; Blood Group A, Blood Group B, and Blood Group O (H) on 6 different core structures (total 18 unique glycans).

Heimburg-Molinaro J, Song X, Smith DF, Cummings RD. Preparation and analysis of glycan microarrays. Curr Protoc Protein Sci. 2011 Apr;Chapter 12:Unit12.10. doi: 10.1002/0471140864.ps1210s64.

Liu Y, McBride R, Stoll M, Palma AS, Silva L, Agravat S, Aoki-Kinoshita KF, Campbell MP, Costello CE, Dell A, Haslam SM, Karlsson NG, Khoo KH, Kolarich D, Novotny MV, Packer NH, Ranzinger R, Rapp E, Rudd PM, Struwe WB, Tiemeyer M, Wells L, York WS, Zaia J, Kettner C, Paulson JC, Feizi T, Smith DF. The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data. Glycobiology. 2016 Nov 22.

Jeyakanthan M, Meloncelli PJ, Zou L, Lowary TL, Larsen I, Maier S, Tao K, Rusch J, Chinnock R, Shaw N, Burch M, Beddows K, Addonizio L, Zuckerman W, Pahl E, Rutledge J, Kanter KR, Cairo CW, Buriak JM, Ross D, Rebeyka I, West LJ. ABH-Glycan Microarray Characterizes ABO Subtype Antibodies: Fine Specificity of Immune Tolerance After ABO-Incompatible Transplantation. Am J Transplant. 2016 May;16(5):1548-58. doi: 10.1111/ajt.13625.