Our ThermoFisher (TFS) Helios 5 Hydra plasma Focused Ion Beam – Scanning Electron Microscope (pFIB-SEM) has arrived at Emory. We are currently coordinating with TFS to schedule its installation, following the completion of room modifications and pre-installation procedures.

The pFIB-SEM technology will allow biomedical investigators in human health and human diseases to understand the three-dimensional organization of cellular environments and have access to research techniques currently not available at Emory University or the region. This instrument will also be equipped with a Delmic Meteor integrated Fluorescent Light Microscope (iFLM).

With this new technology, the IEMC will support structural research of cells and macromolecular complexes involved in infectious diseases, neurodegenerative disorders, heart and liver conditions, and cancer. Acquisition of this pFIB-SEM equipment with cryo-capabilities will have an immediate and profound impact by supporting a number of NIH-sponsored investigators at Emory University and members of the Georgia Core Facilities Partnership, who enjoy shared-access to each other’s state-of-the-art core facilities.

Electron Microscopy Events

Fall 2024 Tomography and Cryo-FIB Workshop

Hosted by the Midwest Center for Cryo-Electron Tomography (MCCET) at UW-Madison, this workshop will be held from September 23-27, 2024.Link

Training topics will include:

  • Introduction to cryo-ET including the basic principles of dose tolerance, illumination, and tilt series collection parameters. 
  • Training in data acquisition workflows that include screening, data collection, and flexible scripting.
  • Training in basic data processing including pre-processing, tomogram generation, and considerations for segmentation and sub-tomogram averaging. 
  • Instruction in 2D/3D correlative imaging workflows using the Leica widefield and cryo-confocal microscopes, and the integrated fluorescence microscope in the Aquilos 2. 
  • Guidance in FIB-milling with AutoTEM and advanced FIB workflows such as waffle milling and lift-out methods. 

Microscopy and Microanalysis 2024 

The meeting of the Microscopy Society of America will take place in Cleveland, OH from July 28th to August 1st, 2024. Link

European Microscopy Congress 2024

This international event will take place in Copenhagen, Denmark from August 25th to the 30th, 2024. Link

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