Images from IEMC-supported publications
Electron micrographs used for these figures were acquired at the IEMC. Thank you for acknowledging our contribution.
Microscopic analysis of R6P6/P6E4 tubes. (a) Stained TEM image of R6P6/P6E4 tubes (1 mg/mL) assembled in TAPS buffer (20 mM, pH 8.0). (b) High-magnification TEM image revealing the lamellar structure that comprises the tube walls. (c) SEM images of R6P6/P6E4 tubes. AFM (d) height trace and (e) amplitude trace of R6P6/P6E4 tubes deposited onto mica. Inset scale bar = 1 μm. (f) 3D reconstruction from AFM data revealing the change in height along the tube shown in the inset of (e). (g) AFM height trace along the tube shown in (f). (h) Synchrotron SAXS/WAXS scattering profile of R6P6/P6E4 tubes (2 mg/mL) in TAPS buffer (20 mM, pH 8.0).
Merg AD, Touponse G, van Genderen E, Blum TB, Zuo X, Bazrafshan A, Siaw HMH, McCanna A, Dyer RB, Salaita K, Abrahams JP, Conticello VP. 2020. Shape-Shifting Peptide Nanomaterials: Surface Asymmetry Enables pH-Dependent Formation and Interconversion of Collagen Tubes and Sheets. Journal of the American Chemical Society. doi: 10.1021/jacs.0c08174
a - Schematic domain representation of the human respiratory syncytial virus (RSV) RNA polymerase (L:P complex), with labeled domain boundary (Alignment details in Supplementary Fig. 4). The domains with missing density are colored in gray. b - The SDS-PAGE gel shows the quality of the wild-type (wt) and mutant RSV RNA polymerase, wt L:P, and L(D811A):P, respectively (repeated ≥ 5 times). c - Elution profile of the purified L:P complex on the Superose 6 Increase 10/300 GL size-exclusion column (repeated ≥ 5 times). d - The RNA dependent RNA polymerization assays show that the purified wt polymerase is active and can synthesis RNA with specific RNA templates (lane 6) compared with that of catalytically inactive polymerase L(D811A):P (lane 3). e - Representative 2D class averages from the 200 kV cryo-EM dataset, selected amongst 125 classes. Scale bar: 100 Å. f - The final cryo-EM density map with the model (colored as a) in two orientations.
Cao D, Gao Y, Roesler C, Rice S, D’Cunha P, Zhuang L, Slack J,Domke M, Antonova A, Romanelli S, Keating S, Forero G, Juneja P, Liang B. 2020. Cryo-EM structure of the respiratory syncytial virus RNA polymerase. Nature Communications. 11(1):368
Structural differences in the media of ppargc1a−/- mouse aorta. Aortas of 6-month-old Ppargc1a+/+ (A and B) and Ppargc1a± (C and D) male mice were fixed, stained with 0.5% aqueous Toluidine Blue O and photographed at low (A and C) and high (B and D) magnification. Dark blue lines of elastic lamina are indicated with arrowheads in A and C. VSMCs in B and D were traced to indicate that more extracellular material is present in Ppargc1a± samples. TEM images of VSMCs in the media of Ppargc1a+/+ (E) and ppargc1a−/- (F and G) aortas were acquired using a Hitachi H-7500 electron microscope. Quantification of EM for both genotypes is shown in H. EM, extracellular matrix; N, nucleus; EL, elastic lamina. Bar: 50 μm in A and C, 10 μm in B and D, 2 μm in E-G.
Salazar G, Cullen A, Huang J, Zhao Y, Serino A, Hilenski L, Patrushev N, Forouzandeh F, Hwang HS. 2019. SQSTM1/p62 and PPARGC1A/PGC-1alpha at the interface of autophagy and vascular senescence. Autophagy. 28:1-19
Negative stain single particle electron microscopy of Them1. A-B. DNterm-Them1 purifies as
a homogenous trimeric complex (~196.5 kD) as determined by size exclusion chromatography (A) and
analytical ultracentrifugation (B). A. Size exclusion chromatography of DNterm-Them1 (blue) and
standards (dotted line). B. c(s) distribution from sedimentation velocity analytical ultracentrifugation of
DNterm-Them1 complex. C. One image of DNterm-Them1 stained with uranyl formate spread across
carbon coated Cu mesh grid collected on Talos 120 C Microscope at a magnification of 96,000X. D. 2D
Class averages of Them1 using Relion 3.0. E. Comparison of 2D class averages (left) and reprojections
(right) of 3D model generated with C3 symmetry to match the class average. Red rectangles highlight
when reprojections of 3D model do not match class averages.
Tillman MC, Imai N, Li Y, Khadka, M, Okafor CD, Juneja P, Adhiyaman A, Hagen SJ, Cohen DE, Ortlund EA. 2020. Allosteric regulation of thioesterase superfamily member 1 by lipid sensor domain binding fatty acids and lysophosphatidylcholine. Proceedings of the National Academy of Sciences USA. Aug 20;202003877
Research Supported by the RPAIEMC
The laboratory of Yonggang Ke at Georgia Tech just published their research on Iron Oxide Nanoparticle assembly using DNA origami. Follow the link to read their exciting results and look at some of their images collected at Emory's RPAIEMC: Programmable Assembly of Iron Oxide Nanoparticles Using DNA Origami
The structure of the respiratory syncytial virus RNA polymerase has been solved by the Liang group at Emory using single particle cryo-EM, with data collected with the Talos Arctica in our EM core:
Cryo-EM structure of the respiratory syncytial virus RNA polymerase
Research from the Schinazi group on Hepatitis B virus now published on Antimicrobial Agents and Chemotherapy, with TEM data acquired at the RPAIEMC:
Novel Hepatitis B Virus Capsid Assembly Modulator Induces Potent Antiviral Responses in Vitro and in Humanized Mice
Results from a collaborative work between Emory University, UC Irvine, and Florida State University on factors that stimulate senescence are now available in Autophagy. TEM data was acquired at the RPAIEMC:
SQSTM1/p62 and PPARGC1A/PGC-1alpha at the interface of autophagy and vascular senescence
Electron Microscopy Events
Cryo-Electron Tomography virtual course
Hosted by the University of Leeds ABSL, eBIC Diamond Light Source, and the University of Leicester, this four-day course is targeted at those with no or minimal experience in cryo-electron tomography. It will introduce participants to sample preparation, tilt-series acquisition, and image analysis pipelines. Participants will work in small groups with instructors to gain hands-on TEM (Titan Krios) experience and learn how to independently use software packages (IMOD, AreTomo, EMAN2, and RELION) for tomogram reconstruction, tomogram segmentation, particle picking, and sub-tomogram averaging. Link
On The Scope – A Masterclass in Practical Scanning Electron Microscopy
This is a training program on the fundamentals of SEM operation. It will take place in Columbus, OH in May of 2024. Link
Lehigh Microscopy School 2024
There will be an expanded list of courses for the 2024 Lehigh Microscopy School (Lehigh University, Bethlehem, PA) from June 2nd to June 7th. The program will focus on SEM and EDS. Other courses will include TEM and FIB. Course information will be found in this Link.
Microscopy and Microanalysis 2024
The meeting of the Microscopy Society of America will take place in Cleveland, OH from July 28th to August 1st, 2024. Link
European Microscopy Congress 2024
This international event will take place in Copenhagen, Denmark from August 25th to the 30th, 2024. Link