Microscopes in Whitehead Biomedical Research Building

Abberior Facility Line | inverted STED confocal | WBRB

  • Super-resolution in 2D/3D via Stimulated Emission Depletion 
  • Four excitation laser lines: 405nm, 485nm, 561nm, 640nm
  • Two pulsed STED lasers: 595nm and 775nm
  • Spectrally tunable emission collection
  • Z-stacks, timelapse, multi-point imaging, tiling, FLIM
  • Live-cell imaging with stage top incubator
  • Inverted microscope body (Olympus IX83)
Abberior Facility Line STED

Nikon A1R HD25 | inverted confocal | WBRB

  • 25mm FOV confocal imaging
  • Six laser lines [nm]: 405, 445, 488, 514, 560, 640
  • Z-stacks, timelapse, multi-point imaging, tiling
  • Live-cell imaging with stage top incubator
  • Galvano and 8 kHz resonant scanners for faster and gentler live-cell imaging
  • FRAP, FLIP, FRET, photoactivation, reflection
  • Inverted microscope body (Nikon Ti2-E)
Nikon A1R HD25

Leica SP8 MP | upright confocal multiphoton | WBRB

  • Point scanning confocal with multiphoton capabilities
  • Eight laser lines [nm]: 458, 476, 488, 496, 514, 561, 594, 633
  • Coherent Chameleon Vision II laser: tunable from 680 to 1080 nm
  • Z-stacks, timelapse, multi-point imaging, tiling
  • Live-cell / animal imaging
  • Galvano and 12 kHz resonant scanners for faster and gentler live-cell imaging
  • 25X/1.0 objective for CLARITY treated specimens
  • Multi-immersion 20x/0.75 objective
Leica SP8 MP

Nikon N-SIM | inverted SIM TIRF-SIM widefield | WBRB

  • Super-resolution achieved with Structured Illumination Microscopy (SIM)
  • Two laser lines [nm]: 488, 561
  • Live-cell imaging with 5% CO2 and temperature control
  • 2D-SIM, 3D-SIM, TIRF-SIM
  • Highly sensitive EMCCD - Andor iXon3 DU-897E
N-SIM

Olympus FV1000 | inverted confocal | WBRB

  • Confocal imaging on an inverted Olympus IX81 body
  • Five laser lines [nm]: 405, 488, 515, 543, 635
  • Three PMTs for fluorescence + another for transmitted light
  • Z-stacks, timelapse, tiling
  • FRAP, FLIP, FRET, photoactivation
Olympus FV1000 inverted (WBRB)

[!jumbovwbrbup!]

BioTek Lionheart FX | inverted widefield | WBRB

  • Automated widefield imaging system
  • Six filtercubes: DAPI, CFP, GFP, YFP, Texas Red, Cy5
  • Fluorescence, bright field, color bright field, phase 
  • Live-cell imaging with temperature control as well as a CO2 and O2 gas controller
  • Reads slides, dishes, and 6 - 1536 well plates
BioTek Lionheart FX